A-type copyright (ATC) proteins are proposed to function in the biogenesis of Fe-S clusters, although their exact role remains controversial.The genome of Mycobacterium smegmatis encodes The impact of fintech development on the entrepreneurial behavior of farmers: evidence from the China Household Finance Survey a single ATC protein, MSMEG_4272, which belongs to the HesB/YadR/YfhF family of proteins.Attempts to generate an MSMEG_4272 deletion mutant by two-step allelic exchange were unsuccessful, suggesting that the gene is essential for in vitro growth.CRISPRi-mediated transcriptional knock-down of MSMEG_4272 resulted in a growth defect under standard culture conditions, which was exacerbated in mineral-defined media.
The knockdown strain displayed reduced intracellular iron levels under iron-replete conditions and increased susceptibility to clofazimine, 2,3-dimethoxy-1,4-naphthoquinone (DMNQ), and isoniazid, while the activity of the Fe-S containing enzymes, succinate dehydrogenase, and aconitase were not affected.This study suggests that MSMEG_4272 plays a role in the regulation of intracellular iron levels Flexible design of Park-and-Ride facility in the city center proximity and is required for in vitro growth of M.smegmatis, particularly during exponential growth.